tetra-primer arms pcr and its applications in genotyping point mutations and single nucleotide polymorphisms
نویسندگان
چکیده
abstract
منابع مشابه
Genotyping single nucleotide polymorphisms in barley by tetra-primer ARMS-PCR.
Single nucleotide polymorphisms (SNPs) are the most abundant form of DNA polymorphism. These polymorphisms can be used in plants as simple genetic markers for many breeding applications, for population studies, and for germplasm fingerprinting. The great increase in the available DNA sequences in the databases has made it possible to identify SNPs by "database mining", and the single most impor...
متن کاملA Novel Multiplex Tetra-Primer ARMS-PCR for the Simultaneous Genotyping of Six Single Nucleotide Polymorphisms Associated with Female Cancers
BACKGROUND The tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) is a fast and economical means of assaying SNP's, requiring only PCR amplification and subsequent electrophoresis for the determination of genotypes. To improve the throughput and efficiency of T-ARMS-PCR, we combined T-ARMS-PCR with a chimeric primer-based temperature switch PCR (TSP) strategy, and used capil...
متن کاملA new tetra-primer ARMS-PCR for genotyping bovine kappa-casein polymorphisms.
Kappa-casein (κ-casein) is one of the most abundant milk proteins. Its main function is to avoid the aggregation of casein micelles, keeping them, and therefore calcium phosphate, in pockets in solution. In bovines, a κ-casein functional polymorphism has been associated with fat, calcium, and protein milk contents and faster curd contraction in cheese production. Quicker curd contraction reduce...
متن کاملDesigning, optimization and validation of tetra-primer ARMS PCR protocol for genotyping mutations in caprine Fec genes
New, quick, and inexpensive methods for genotyping novel caprine Fec gene polymorphisms through tetra-primer ARMS PCR were developed in the present investigation. Single nucleotide polymorphism (SNP) genotyping needs to be attempted to establish association between the identified mutations and traits of economic importance. In the current study, we have successfully genotyped three new SNPs ide...
متن کاملPrimer1: Primer Design Web Service for Tetra-Primer ARMS-PCR
Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of mismatch bases. The optimal selection of primers can be achieved in an automated way using a program which evaluates candidate primers for a given...
متن کاملDetection of Single-Nucleotide Polymorphism in the Bovine AGPAT6 Gene Associated with Milk Fat Content using Tetra-Primer ARMS PCR-based Assay in Karan Fries Breeding Bulls
Background: The bovine AGPAT6 gene is one of the potential candidate genes governing milk fat synthesis.Objectives: Identification of single nucleotide polymorphisms (SNP) in the targeted region of AGPAT6 gene and their effect on expected breeding values (EBV) of first lactation milk production traits viz. fat %, fat yield and 305 days milk yield in Karan Fries (KF) breeding bulls were so...
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عنوان ژورنال:
basic and clinical cancer researchجلد ۴، شماره ۳&۴، صفحات ۲۳-۳۳
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